Does your cfDNA extraction process affect the Limit of Detection (LOD) of a particular gene target? Our new products, cfDNA Extraction Sensitivity Panels and Extraction Low Positive Controls, can help your research and development for liquid biopsy assays.
Build your own controls using your high-positive samples to save time. With our DNA-depleted human plasma or serum as a matrix, you can produce reference materials specific to your time-sensitive needs while retaining the natural components of the sample.
The Plasma Genomic cfDNA Extraction Control is for the purpose of monitoring the degree of genomic DNA (gDNA) contamination in the extracted cfDNA. DNA-free plasma is spiked with either with high molecular weight gDNA molecules or 170 bp cfDNA fragments or both.
Buffer-based or plasma-based controls are produced by mixing single or multiplexed mutant gene fragments with a native, whole-genome cfDNA background (Figure B).