Buffer-based or plasma-based controls are produced by mixing single or multiplexed mutant gene fragments with a native, whole-genome cfDNA background (Figure B).
Anchor Molecular manufactures two types of products: Mutation Quantification Control and Mutation Reference Standard. The user can quantify either the absolute copy numbers or the relative number of the mutant gene fragments.
AM Multiplexed Mutation Quantification Control
- Function as both positive and negative controls
- For use in daily assays
- AF% determined based on average wild-type copy numbers
- Customized controls available upon request
- Price upon request
This product is used as Positive and Negative Controls in daily assays. The AF% is determined based on average wildtype copy numbers. It includes all mutant genes listed in the table below. Custom products with different parameters are available upon request.
| Cat. No. | Base Matrix (vol) | Allele Frequency (%) | Wildtype gDNA (ng) |
| 60100101 | Buffer (25 ul) | 0 or 2 | 250 |
| 60100102 | EDTA Plasma (5 ml) | 0 or 2 | 50 |
| 60100103 | EDTA Plasma (5 ml) | 0 or 2 | 25 |
AM Multiplexed Mutation Reference Standard
- Highest quality reference standards
- For use in research and assay development
- Mutant genes at fixed allele frequency (AF%) determined by ddPCR
- Customized reference standards available upon request
- Price upon request
Mutant genes are made at fixed allele frequency (AF%) determined by ddPCR. The product includes all mutant 37 mutant genes listed in red in the table below. Custom products with different parameters are available upon request.
| Cat. No. | Base Matrix (vol) | Allele Frequency (%) | Wildtype gDNA (ng) |
| 60100201 | Buffer (25 ul) | 0, 0.1, 0.3, 1, or 2 | 250 |
| 60100202 | EDTA Plasma (5 ml) | 0, 0.1, 0.3, 1, or 2 | 125 |
Mutation List
| EGFR (T790M) | KRAS (G12S) | TP53 (R175H) | EGFR (D770-N771insG) |
| EGFR (C797S) | KRAS (G12A) | APC (R1450X) | EGFR (delE746-A750) |
| EGFR (T790M & C797S cis) | KRAS (G12R) | Beta2-M | EGFR (delL747-T751insS) |
| EGFR (G719S) | KRAS (G13D) | DDR2 | EGFR (delL747-P753insS) |
| EGFR (G719C) | BRAF (V600E) | LKB1 | |
| EGFR (G719A) | BRCA1 (P871L) | MDM2 | |
| EGFR (S720F) | BRCA2 (N372H) | MDM4 | EML4-ALK v1 |
| EGFR (L858R) | ERBB2 (S310F) | POLE | EML4-ALK v2 |
| EGFR (L861Q) | JAK2 (V617F) | PTEN | EML4-ALK v3 |
| EGFR (L861R) | MAP2K1 (P124S) | SKT11 | CCDC6-RET |
| KRAS (G12D) | MET (Y1253D) | NCOA4-RET | |
| KRAS (G12V) | PIK3CA (H1047R) | EGFR (D770-N771insNPG) | CD74-ROS1 |
| KRAS (G12C) | TERT (A305A) | EGFR (D770-N771insSVQ) | SLC34A2-ROS1 |