Build your own controls using your high-positive samples to save time. With our DNA-depleted human plasma or serum as a matrix, you can produce reference materials specific to your time-sensitive needs while retaining the natural components of the sample.
Prefer to have a knowledgeable lab do the work? Send your plasma to Anchor Molecular and we’ll remove the DNA background for you.
|Product Name||Cat. No.||Size|
|DD Plasma (DNA-Depleted Plasma)||60601001||50, 200, 500, 1000 ml|
|DD Serum (DNA-Depleted Serum)||60601002||50, 200, 500, 1000 ml|
Why Is Plasma Better Than Buffer As Reference Material Matrix?
A plasma matrix is a better representation of a real patient sample because the natural, interfering proteins are retained. This allows for a more realistic process in your quality system.
Equal amounts of DNA fragments were spiked into TE Buffer (blue) and human plasma (red).
While the 170 bp peak was recovered similarly in each matrix, much more genomic DNA was extracted from the plasma than from the TE buffer resulting in a more patient-like matrix.
See Figure A.
|Concentration of DNA: < 0.02 ng/ml|
|Total protein: 5-7 g/dL|
|Immunoglobulin G: 500-1700 mg/dL|
|Immunoglobulin A: 90-400 mg/dL|
|Immunoglobulin M: 20-172 mg/dL|
|Cholestrol, Total: 50-199 mg/dL|
|Triglycerides: 50-149 mg/dL|
|HDL Cholestrol: > 20 mg/dL|
|VLDL Cholestrol: 10-40 mg/dL|
|LDL Cholestrol: 20-99 mg/dL|
|Tested Negative for HIV, HBV, HCV,|
|HTLV and Syphilis by FDA approved methods|