The Plasma Genomic cfDNA Extraction Control is for the purpose of monitoring the degree of genomic DNA (gDNA) contamination in the extracted cfDNA. DNA-free plasma is spiked with either with high molecular weight gDNA molecules or 170 bp cfDNA fragments or both.

Why Extraction Control?

The extraction of cfDNA from patient plasma is a complicated process that causes significant variability from run to run, kit to kit, and operator to operator. Contaminated large-sized genomic DNA interferes with the mutation quantification of cfDNA, which is mainly ~170 bp in size.

Some extraction kits are better in preferentially extracting the 170bp. Figure 4 shows the cfDNA extraction Kit #1 is much better in eliminating the gDNA contamination while maintaining superior extraction yield in 170 bp DNA fragments.

Product Name Cat. No. DNA in 5ml plasma
Positive Control Plasma 1: high MW gDNA + 170 bp fragments 60100001 10ng gDNA, 5ng 170bp
Positive Control Plasma 2:  high MW gDNA 60100002 10ng gDNA
Positive Control Plasma 3:  170 bp fragments 60100003 5ng 170bp
Negative Control Plasma 60601012 0ng

AM cfDNA Extraction Sensitivity Panels

  • Monitors plasma cfDNA loss
  • Two Types of Sensitivity Panels:
    • Panel 1
      • for absolute copies/mL
      • DNA-depleted plasma spiked with specified 170bp DNA fragment
      • increasing copies/mL
      • constant 5ng/mL fragmented gDNA background
    • Panel 2
      • for gDNA pg/mL
      • DNA-depleted plasma spiked with ~170bp gDNA fragments
      • 0, 30, 100, 300, 1,000, 3,000 pg/mL fragment concentrations
  • Standardizes quality control workflow for plasma cfDNA extraction
  • Assays for panels sold separately
  • Customized sensitivity panels available upon request
  • Price upon request

AM cfDNA Extraction Low Positive Controls

  • Determines minimum extractions of cfDNA copies
  • Two Types of Low Positive Controls:
    • Mutant Low Positive Control
      • 50 copies/mL of specified DNA
      • 5ng/mL gDNA at ~170bp
    • gDNA Low Positive Control
      • 100pg/mL gDNA fragments
  • Standardizes quality control workflow for plasma cfDNA extraction
  • Assays for panels sold separately
  • Customized extraction controls available upon request
  • Price upon request

AM cfDNA Extraction Specificity Controls

  • Screens high molecular weight and DNA fragment loss
  • Four types of specificity controls:
    • Positive Control Plasma 1 – High MW gDNA +170bp fragments
    • Positive Control Plasma 2 – High MW gDNA only
    • Positive Control Plasma 3 – 170bp fragments only
    • Negative Control Plasma
  • Price upon request