The Plasma Genomic cfDNA Extraction Control is for the purpose of monitoring the degree of genomic DNA (gDNA) contamination in the extracted cfDNA. DNA-free plasma is spiked with either with high molecular weight gDNA molecules or 170 bp cfDNA fragments or both.
Why Extraction Control?
The extraction of cfDNA from patient plasma is a complicated process that causes significant variability from run to run, kit to kit, and operator to operator. Contaminated large-sized genomic DNA interferes with the mutation quantification of cfDNA, which is mainly ~170 bp in size.
Some extraction kits are better in preferentially extracting the 170bp. Figure 4 shows the cfDNA extraction Kit #1 is much better in eliminating the gDNA contamination while maintaining superior extraction yield in 170 bp DNA fragments.
|Product Name||Cat. No.||DNA in 5ml plasma|
|Positive Control Plasma 1: high MW gDNA + 170 bp fragments||60100001||10ng gDNA, 5ng 170bp|
|Positive Control Plasma 2: high MW gDNA||60100002||10ng gDNA|
|Positive Control Plasma 3: 170 bp fragments||60100003||5ng 170bp|
|Negative Control Plasma||60601012||0ng|