cfDNA Extraction Specificity Controls

The Plasma Genomic cfDNA Extraction Control are human DNA-free plasma spiked with cfDNA at different sizes. It is for the purpose of monitoring the degree of genomic DNA (gDNA) contamination in the extracted cfDNA. DNA-free human plasma is spiked with either with high molecular weight gDNA molecules or 170 bp cfDNA fragments or both.

Why Extraction Control?

The extraction of cfDNA from patient plasma is a complicated process that causes significant variability from run to run, kit to kit, and operator to operator. Contaminated large-sized genomic DNA interferes with the mutation quantification of cfDNA, which is mainly ~170 bp in size. Some extraction kits are better in preferentially extracting the 170bp. Figure 4 shows the cfDNA extraction Kit #1 is much better in eliminating the gDNA contamination while maintaining superior extraction yield in 170 bp DNA fragments.   AM cfDNA Extraction Specificity Controls

• Screens high molecular weight and DNA fragment loss
• Four types of specificity controls:
  • Positive Control Plasma 1 – High MW gDNA +170bp fragments
  • Positive Control Plasma 2 – High MW gDNA only
  • Positive Control Plasma 3 – 170bp fragments only
  • Negative Control Plasma
• Price upon request

 

Product Name	Cat. No.	DNA in 5ml plasma
Positive Control Plasma 1: high MW gDNA + 170 bp fragments	60100001	10ng gDNA, 5ng 170bp
Positive Control Plasma 2:  high MW gDNA	60100002	10ng gDNA
Positive Control Plasma 3:  170 bp fragments	60100003	5ng 170bp
Negative Control Plasma	60601012	0ng