Multiplexed ctDNA fragments (~150bp) mixed with nucleosomally fragmented wildtype cfDNA background in human plasma. The cell-derived ctDNA fragments are generated by Anchor’s unique multiplexed gene-editing method and are nucleosomally fragmented to around 150bp. The cell-derived variants are suitable for both the amplicon-based and capture-based methods. The synthetic ctDNA fragments are gBlocks-like dsDNA of 150-170bp and are suitable for amplicon-based applications, including ddPCR, qPCR, and amplicon-based NGS.
Please note:
1. The gBlock-like synthetic variants, since their mutation is in the middle of the fragment rather than randomly distributed, generally have lowered AF% due to our value-assignment methods based on ddPCR (and amplicon-based NGS). Customers using capture-based assay will receive a 20% discount if notify us during ordering.
2. The fusions carry breakpoints within exons of fusion partners and are for demonstration purpose.
3. For custom SNV/indel/fusion variants for either cfDNA assays or solid tumor tests, please request by clicking the “Order Here” button and select the “Custom Molecular Controls” option.
| Cat. No. | Base Matrix | Allele Frequency(%) | Total gDNA(ng) |
|---|---|---|---|
| 60100101 | Buffer (15ul) | 3% | 300 |
| 60100102 | EDTA Plasma (5ml) | 3% | 125 |
The standard includes 50+ Variants listed in tables below based on the source of DNA:
| Cell Derived Variant | |||
|---|---|---|---|
| APC Q1429* | EGFR E746-A750del v1&v2 | KIT D816V | PIK3CA E545K |
| APC R1450* | EGFR L858R | KRAS G12C | PIK3CA H1047R |
| BRAF V600E | EGFR T790M | KRAS G12D | PTEN R130G |
| CTNNB1 T41A | FGFR3 S249C | KRAS G12V | TP53 R175H |
| EGFR C797S | IDH1 R132H | NRAS Q61R | TP53 R248Q |
| EGFR D770_N771insG | |||
| Synthetic Variant | |||
| AKT1 E17K | HRAS Q61R | CCDC6-RET fusion | |
| ATM R337C | KIT W557_K558del | NOTCH1 H1601L | CD74-ROS1 fusion |
| CDKN2A R80* | MAP2K1 P124S | PDGFRA D842V | EML4-ALK v1 fusion |
| DDR2 R709* | MDM2 R189H | POLE P286R | ETV6-NTRK3 fusion |
| EGFR G719A | MDM4 Q118L | SMARCA4 T910M | FGFR2-BICC1 fusion |
| ERBB2 S310F | MED12 G44D | STK11 F354L | KIF5B-RET fusion |
| ERBB4 R711G | MET Y1253D | TP53 R273H | SLC34A2-ROS1 fusion |
| ESR1 D538G | |||
