ctDNA Standards for Amplicon-based Assays

Multiplexed ctDNA fragments (~150bp) mixed with nucleosomally fragmented wildtype cfDNA background in human plasma. The cell-derived ctDNA fragments are generated by Anchor’s unique multiplexed gene-editing method and are nucleosomally fragmented to around 150bp. The cell-derived variants are suitable for both the amplicon-based and capture-based methods. The synthetic ctDNA fragments are gBlocks-like dsDNA of 150-170bp and are suitable for amplicon-based applications, including ddPCR, qPCR, and amplicon-based NGS.

Please note:
1. The gBlock-like synthetic variants, since their mutation is in the middle of the fragment rather than randomly distributed, generally have lowered AF% due to our value-assignment methods based on ddPCR (and amplicon-based NGS). Customers using capture-based assay will receive a 20% discount if notify us during ordering.
2. The fusions carry breakpoints within exons of fusion partners and are for demonstration purpose.
3. For custom SNV/indel/fusion variants for either cfDNA assays or solid tumor tests, please request by clicking the “Order Here” button and select the “Custom Molecular Controls” option.

The standard includes 50+ Variants listed in tables below based on the source of DNA:

Sample Testing Data in CoA
cfDNA Characterization