DNA-Depleted Plasma
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Cell-Free DNA (cfDNA) Analysis: Ideal for liquid biopsy research in cancer diagnostics and monitoring, DNA-depleted plasma ensures that only cfDNA is detected, significantly improving assay accuracy.
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Assay Development and Validation: Ideal matrix for liquid biopsy assay development in cancer diagnostics and monitoring, DNA-depleted plasma ensures that cfDNA is detected with authentic accuracy, precision and assay linearity in complicated human plasma environment.
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Pathogen DNA Detection: Crucial for detecting true copies of viral or bacterial DNA in plasma matrix, DNA-depleted plasma reduces the risk of false positives caused by background contaminating DNA from unknown . Crucial for detecting true copies of viral or bacterial DNA in plasma matrix, DNA-depleted plasma reduces the risk of false positives caused by background contaminating DNA from unknown .
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Quality Control: Used to create reference materials that mimic patient samples without the variability introduced by background DNA, ensuring consistent and reliable performance of diagnostic tests.
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Research on Host-Pathogen Interactions: Facilitates studies focusing on the interactions between host and pathogen DNA by clearly distinguishing between the two, leading to more precise research outcomes.
| Cat. No. | Product Name | Base Matrix | Price | Quantity | |
|---|---|---|---|---|---|
| 60601001 | DD Plasma (1000 ml) | 1000 ml | Login for Price | ||
| 60601002 | DD Plasma (500 ml) | 500ml | Login for Price | ||
| 60601003 | DD Plasma (100 ml) | 100ml | Login for Price | ||
| 60601004 | DD Plasma (50 ml) | 50ml | Login for Price | ||
| 60601005 | DD Plasma (40 vials) | 40 vials, 5mls per vial | Login for Price |
Build your own controls using your high-positive samples to save time. With our DNA-depleted human plasma (DNA-free plasma) as a matrix, you can produce reference materials specific to your time-sensitive needs while retaining the natural components of the sample. The DNA-depleted plasma is made from a pool of plasma from at least 30 patients.
Why Use DNA-Depleted EDTA Plasma?
(download document)
Characterization of DNA-Depleted Plasma
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- Highest quality DNA-depleted human plasma
- For use in research, assay development, & daily assays
- Retains naturally-occurring, interfering proteins
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- Allows for more realistic process in quality systems
- Customized plasma available upon request
- Price upon request
- Less processed plasma (more DNA: 20-100 pg/ml or higher) is available upon request
| Chemical Product Characteristics |
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| Concentration of Human gDNA: < 20 pg/mL |
| Total protein: 5-7 g/dL |
| Immunoglobulin G: 500-1700 mg/dL |
| Immunoglobulin A: 90-400 mg/dL |
| Immunoglobulin M: 20-172 mg/dL |
| Cholesterol, Total: 50-199 mg/dL |
| Triglycerides: 50-149 mg/dL |
| HDL Cholesterol: > 20 mg/dL |
| VLDL Cholesterol: 10-40 mg/dL |
| LDL Cholesterol: 20-99 mg/dL |
| pH: 7.2-7.6 |
| Tested Negative for HIV, HBV, HCV, |
| HTLV and Syphilis by FDA approved methods |
Exosome counts in DNA-depleted plasma are normal compared to regular plasma.
Why Is Plasma Better Than Buffer As Reference Material Matrix?
A plasma matrix is a better representation of a real patient sample because the natural, interfering proteins are retained. This allows for a more realistic process in your quality system.
Equal amounts of DNA fragments were spiked into TE Buffer (blue) and human plasma (red).
While the 170 bp peak was recovered similarly in each matrix, much more genomic DNA was extracted from the plasma than from the TE buffer resulting in a more patient-like matrix.
See Figure A.
A significant portion of florescence signal actually comes from protein contamination
Documents
Sample Certification (COA)
(The COA for your order will be enclosed within the shipping package.)
| Lot # | Certificate Type | Catalog Number(s) |
|---|---|---|
| 240636 | Certificate of Analysis | 60601001 |
