Usage Condition: The 3 ml sample is used the same way as a patient plasma sample.
Storage Condition: 2-8°C for short term (<60 days), -20oC for long term (> 60 days).
Shipping Condition: Blue Ice.
Shelf Life: Three years from the date of receipt under proper storage condition.
The Extraction Controls are either ctDNA spiked in plasma at a fixed allele frequency (Panel 1) or wild-type cfDNA spiked in plasma at a fixed concentration (Panel 2). They are used as controls to help monitor cfDNA extraction efficiency from real human plasma, determine how low the absolute number of copies can be reliably extracted, and standardize the QC workflow for the cfDNA extraction process.
cfDNA fragments (~170bp) are mixed with full length genomic DNA background in real human plasma. The control is used for evaluating size specificity of cfDNA extraction by the extraction kit or method. A good cfDNA extraction method should preferentially extract the 170bp cfDNA only while excluding the full length chromosomal DNA.
DNA-depleted human plasma is essential in various scenarios where the presence of background DNA could interfere with the accuracy and reliability of results. Here are some key applications:
Cell-Free DNA (cfDNA) Analysis: Ideal for liquid biopsy research in cancer diagnostics and monitoring, DNA-depleted plasma ensures that only cfDNA is detected, significantly improving assay accuracy.
Assay Development and Validation: Ideal matrix for liquid biopsy assay development in cancer diagnostics and monitoring, DNA-depleted plasma ensures that cfDNA is detected with authentic accuracy, precision and assay linearity in complicated human plasma environment.
Pathogen DNA Detection: Crucial for detecting true copies of viral or bacterial DNA in plasma matrix, DNA-depleted plasma reduces the risk of false positives caused by background contaminating DNA from unknown . Crucial for detecting true copies of viral or bacterial DNA in plasma matrix, DNA-depleted plasma reduces the risk of false positives caused by background contaminating DNA from unknown .
Quality Control: Used to create reference materials that mimic patient samples without the variability introduced by background DNA, ensuring consistent and reliable performance of diagnostic tests.
Research on Host-Pathogen Interactions: Facilitates studies focusing on the interactions between host and pathogen DNA by clearly distinguishing between the two, leading to more precise research outcomes.
Using DNA-depleted plasma in these scenarios enhances the specificity, sensitivity, and overall reliability of molecular testing and research.
